Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles.

TitleAdipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles.
Publication TypeJournal Article
Year of Publication2016
AuthorsFerng AS, Marsh KM, Fleming JM, Conway RF, Schipper D, Bajaj N, Connell AM, Pilikian T, Johnson K, Runyan R, Black SM, Szivek JA, Khalpey Z
JournalSpringerplus
Volume5
Issue1
Pagination2057
Date Published2016
ISSN2193-1801
Abstract

BACKGROUND: Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen.

RESULTS: Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification.

CONCLUSIONS: The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.

DOI10.1186/s40064-016-3712-1
Alternate JournalSpringerplus
PubMed ID27995034
PubMed Central IDPMC5133220
Grant ListP01 HL101902 / HL / NHLBI NIH HHS / United States
P30 CA023074 / CA / NCI NIH HHS / United States
R01 HL060190 / HL / NHLBI NIH HHS / United States
R01 HL067841 / HL / NHLBI NIH HHS / United States